RNA-seq
Science & Engineering abbreviation · Genetics
| Stands for | RNA sequencing |
|---|---|
| Categories | Science & Engineering |
RNA-seq stands for RNA sequencing, a genetics abbreviation.
Use of high-throughput sequencing to measure which genes are expressed in a sample and at what levels, by converting RNA to cDNA and sequencing millions of fragments. RNA-seq also reveals alternative splicing, fusion genes and new transcripts.
Example: RNA-seq identified 1,240 genes differentially expressed between treated and control cells.
Usage notes
RNA-seq became widespread around 2008 and largely replaced gene-expression microarrays. Results are normalized to account for sequencing depth and gene length, with units such as TPM (transcripts per million). Single-cell versions profile individual cells.
Related abbreviations
More genetics abbreviations
- NHEJ – Non-homologous end joining
- ORF – Open reading frame
- PAM – Protospacer adjacent motif
- PRS – Polygenic risk score
- QTL – Quantitative trait locus
- RFLP – Restriction fragment length polymorphism
- RNAi – RNA interference
- scRNA-seq – Single-cell RNA sequencing
- sgRNA – Single guide RNA
- shRNA – Short hairpin RNA
- siRNA – Small interfering RNA
- SNP – Single-nucleotide polymorphism